Introducing a High Throughput Nanozymatic Method for Eco-Friendly Nanozyme-Mediated Degradation of Methylene Blue in Real Water Media

In this work, a high throughput nanozyme-based catalytic system was developed for eco-friendly nanozyme-mediated biodegradation of organic dye, methylene blue, in real water media. The above-mentioned nanozymes were synthesized and then characterized their morphology, composition, size by Transmission Electron Microscopy (TEM), elemental mapping, Dynamic Light Scattering (DLS) analysis, order. nanozymatic properties the as-prepared nanomaterials evaluated standard enzyme activity assay, revealing peroxidase-like performances nanozymes. Therefore, more precise reporting nanozymes, kinetics behavior Lineweaver-Burk method, Vmax as 0.263 µM sec-1 Km low 0.03 mM, efficiency affinity Hence, utilized degradation dye utilizing blue model dye. factors affecting including pH, time, ionic strength, nanozyme amount, etc. optimized one-factor-at-a-time optimization method. At optimal experimental conditions, can degrade about 99.0% within time 7 min. method finally employed from media such pool water, mineral river tap samples. results study revealed an excellent yield over 94.3%-99.0% different samples, proving suitability